According to the invention preparation for regeneration of bone defects is employed in many branches of medicine, especially in orthopedics, dental surgery, reconstructive surgery, periodonthology and implantology. The preparation has been developed on the basis of inorganic chemical compound naturally occurring, i.e. deproteinized human bone, and on the basis of at least one of inorganic chemical compounds synthetically occurring, i.e. bioglass in the form of granulated high calcium product (54% mol. CaO), obtained with the use of zol-gel method in the system CaO—SiO2—P2O5, of density 2.9082 g/cm3, with phase composition with a dominant glassy phase and beginnings of crystallization of apatite, heat treated at temperature of 800° C., having a specific surface BET 57.8166 m2/g and/or tricalcium phosphate—TCP in the form of granulated product with chemical formula Ca3 (PO4)2. As a component deproteinized particles of human bone are used, favorably with granulation 0.3-0.5 mm, bioglass favorably with granulation 0.3-0.5 mm and/or TCP—tricalcium phosphate favorably with granulation 0.3-0.5 mm. The amount of bone material in the preparation amounts favorably to 70-80% of the preparation weight, the amount of bioglass in preparation amounts favorably to 10-15% of the preparation weight, the amount of TCP in the preparation amounts favorably to 10-15% of the preparation weight. During implantation binder in the form of blood from patient's operative wound is introduced into the preparation. Amount of the preparation in mixture favorably amounts to 75-80% of the mixture weight and the amount of own blood favorably amounts to 20-25% of the mixture weight.