Invention Grant
- Patent Title: Methods compositions, and kits for detection of microRNA
- Patent Title (中): 用于检测microRNA的方法,组合物和试剂盒
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Application No.: US11627926Application Date: 2007-01-26
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Publication No.: US08314220B2Publication Date: 2012-11-20
- Inventor: Rebecca L. Mullinax , Joseph A. Sorge
- Applicant: Rebecca L. Mullinax , Joseph A. Sorge
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C07H21/02
- IPC: C07H21/02 ; C12Q1/68 ; C07H21/04

Abstract:
The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise designing mRNA-specific primers, adding a polyA tail to the miRNA, and using reverse transcription and amplification to detect the miRNA. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the methods of the invention.
Public/Granted literature
- US20080182239A1 Methods, Compositions, and Kits for Detection of microRNA Public/Granted day:2008-07-31
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