Methods and systems disclosed herein provide the capability to automatically process digital pathology images quickly and accurately. According to one embodiment, an digital pathology image segmentation task may be divided into at least two parts. An image segmentation task may be carried out utilizing both bottom-up analysis to capture local definition of features and top-down analysis to use global information to eliminate false positives. In some embodiments, an image segmentation task is carried out using a “pseudo-bootstrapping” iterative technique to produce superior segmentation results. In some embodiments, the superior segmentation results produced by the pseudo-bootstrapping method are used as input in a second segmentation task that uses a combination of bottom-up and top-down analysis.