Invention Grant
- Patent Title: Sequence data by reduction of noise due to carry-over primer
- Patent Title (中): 通过降低遗留引物引起的噪音的序列数据
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Application No.: US12935977Application Date: 2009-03-31
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Publication No.: US08354233B2Publication Date: 2013-01-15
- Inventor: Brad T. Cookson , Dhruba J. Sengupta
- Applicant: Brad T. Cookson , Dhruba J. Sengupta
- Applicant Address: US WA Seattle
- Assignee: University of Washington
- Current Assignee: University of Washington
- Current Assignee Address: US WA Seattle
- Agency: Kilpatrick Townsend & Stockton LLP
- International Application: PCT/US2009/039020 WO 20090331
- International Announcement: WO2009/124085 WO 20091008
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The present invention provides methods of reducing the background signal of a nucleic acid sequencing reaction. In particular, the invention provides methods of specifically degrading unwanted chain termination reaction products generated by the extension of primers carried over from the amplification step of the sequencing reaction. These methods are amenable for use with both one step and two-step amplification/chain termination reaction sequencing protocols.
Public/Granted literature
- US20110244452A1 SEQUENCE DATA BY REDUCTION OF NOISE DUE TO CARRY-OVER PRIMER Public/Granted day:2011-10-06
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