Invention Grant
US08357535B2 PURO-DHFR quadrifunctional marker and its use in protein production
有权
PURO-DHFR四功能标记及其在蛋白质生产中的应用
- Patent Title: PURO-DHFR quadrifunctional marker and its use in protein production
- Patent Title (中): PURO-DHFR四功能标记及其在蛋白质生产中的应用
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Application No.: US12601553Application Date: 2008-06-06
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Publication No.: US08357535B2Publication Date: 2013-01-22
- Inventor: Michel Kobr , Philippe Dupraz
- Applicant: Michel Kobr , Philippe Dupraz
- Applicant Address: CH Coinsins, Vaud
- Assignee: Merck Serono SA
- Current Assignee: Merck Serono SA
- Current Assignee Address: CH Coinsins, Vaud
- Agency: Saliwanchik, Lloyd & Eisenschenk
- Priority: EP07109829 20070607
- International Application: PCT/EP2008/057109 WO 20080606
- International Announcement: WO2008/148881 WO 20081211
- Main IPC: C12N15/85
- IPC: C12N15/85 ; C12P21/00
Abstract:
This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).
Public/Granted literature
- US20100167298A1 PURO-DHFR Quadrifunctional Marker and its use in Protein Production Public/Granted day:2010-07-01
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