The present invention discloses a control method for generating varying colored lights in a keyboard and a self-luminous keyboard for realizing the same method. The control method comprises steps: establishing a driving signal table storing a plurality of backlight driving signals; setting the conditions for outputting the backlight driving signals respectively; detecting a keying signal generated by pressing a key; outputting the backlight driving signal corresponding to the keying signal to drive a backlight unit, or sequentially outputting the backlight driving signals according to the keying signal to drive a backlight unit. The self-luminous keyboard for realizing the same method comprises a memory unit storing the driving signal table and a microcontroller unit detecting the keying signals. The microcontroller unit sequentially outputs the backlight driving signals to drive the backlight unit, or outputs the backlight driving signal corresponding to the keying signal to drive the backlight unit.