Disclosed is a release liner having a multilayer structure of at least three layers including two surface layers and an intermediate layer, in which one of the two surface layers contains a high-density polyethylene, the other contains a low-density polyethylene, and the intermediate layer contains a low-density polyethylene alone as its resin component. Also disclosed is a pressure-sensitive adhesive sheet including the release liner and at least one pressure-sensitive adhesive layer, in which a release force X (N/mm) between the release liner and the pressure-sensitive adhesive layer and a rigidity Y of the release liner [(Young's modulus of the release liner)×(thickness)3] (N·mm) satisfy the following conditions: 0