A hydrophobic coating having a contact angle of at least about 150 degrees is disclosed herein, which coating comprises: a polymer substrate comprising a first linker incorporated therein at a specified surface density of from about 3×10−4 to about 9×10−8 units per nm2; a first polymer layer in contact with said first linker; a second linker in contact with said first polymer layer; and a second polymer layer in contact with said second linker. Also disclosed herein are methods for producing the hydrophobic coating. This hydrophobic coating is useful for making microfluidic devices having a hydrophobic coating on the microfluidic channels, as well as other uses in the field of microfluidics.