Invention Grant
US08367383B2 Mutant Δ-5 desaturases mutated in the heme-binding motif (HPGG) and their use in making polyunsaturated fatty acids
有权
在血红素结合基序（HPGG）中突变的突变体和Dgr; -5去饱和酶及其在制备多不饱和脂肪酸中的用途
- Patent Title: Mutant Δ-5 desaturases mutated in the heme-binding motif (HPGG) and their use in making polyunsaturated fatty acids
- Patent Title (中): 在血红素结合基序（HPGG）中突变的突变体和Dgr; -5去饱和酶及其在制备多不饱和脂肪酸中的用途
-
Application No.: US13585411Application Date: 2012-08-14
-
Publication No.: US08367383B2Publication Date: 2013-02-05
- Inventor: Quinn Qun Zhu , Dana M. Walters Pollak
- Applicant: Quinn Qun Zhu , Dana M. Walters Pollak
- Applicant Address: US DE Wilmington
- Assignee: E I du Pont de Nemours and Company
- Current Assignee: E I du Pont de Nemours and Company
- Current Assignee Address: US DE Wilmington
- Main IPC: C12P7/64
- IPC: C12P7/64 ; C12N1/20 ; C12N1/00 ; C12N1/12 ; C12N15/00 ; C07H21/04
Abstract:
The present invention relates to mutant Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid [DGLA; 20:3 ω-6] to arachidonic acid [ARA; 20:4 ω-6] and/or eicosatetraenoic acid [ETA; 20:4 ω-3] to eicosapentaenoic acid [EPA; 20:5 ω-3] and which possess at least one mutation within the HPGG motif of the cytochome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant Δ5 desaturases in oleaginous yeast, are disclosed.
Public/Granted literature
Information query
