Invention Grant
US08367391B2 Method for detecting or determining abnormal prion protein associated with transmissible spongiform encephalopathy in blood-derived specimen or body fluid-derived specimen
失效
用于检测或确定与血源性标本或体液衍生标本中的传染性海绵状脑病有关的异常朊病毒蛋白的方法
- Patent Title: Method for detecting or determining abnormal prion protein associated with transmissible spongiform encephalopathy in blood-derived specimen or body fluid-derived specimen
- Patent Title (中): 用于检测或确定与血源性标本或体液衍生标本中的传染性海绵状脑病有关的异常朊病毒蛋白的方法
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Application No.: US12674857Application Date: 2008-08-22
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Publication No.: US08367391B2Publication Date: 2013-02-05
- Inventor: Kazuo Tsukui
- Applicant: Kazuo Tsukui
- Applicant Address: JP Minato-ku, Tokyo
- Assignee: Japanese Red Cross Society
- Current Assignee: Japanese Red Cross Society
- Current Assignee Address: JP Minato-ku, Tokyo
- Agency: Merchant & Gould P.C.
- Priority: JP2007-217203 20070823
- International Application: PCT/JP2008/065470 WO 20080822
- International Announcement: WO2009/025396 WO 20090226
- Main IPC: C12N7/02
- IPC: C12N7/02 ; C12P21/06 ; C07K1/30 ; C12Q1/70
Abstract:
A pretreatment method of a specimen used for detecting or determining abnormal prion protein (PrPres) associated with transmissible spongiform encephalopathy (TSE), wherein (1) a specimen which had been treated with proteinase K is heated in the presence of sodium dodecyl sulfate (SDS) to dissolve proteins and inactivate infectious activity in the specimen at the same time; (2) the specimen processed in the above (1) is cooled under a neutral condition to make abnormal prion protein (PrPres) associated with transmissible spongiform encephalopathy (TSE) aggregated; (3) the aggregate formed in the above (2) is separated from a solution; and (4) the separated PrPres aggregate is detected by the ultrasensitive chemiluminescence method.
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