A mouse with a shooting function and an image pickup system are provided. The image pickup system includes a mouse and a computer system. A shooting program is installed in the computer system. The mouse includes an image pickup device and a supporting member. The supporting member is used for fixing a business card. The shooting program is executed to judge whether the business card is separated from the image pickup device by a shooting distance, which is equal to a focal length of the image pickup device. If the business card is separated from the image pickup device by the shooting distance, the image pickup device is enabled by the shooting program to shoot the business card, thereby acquiring a business card image.