A cosmetic supply organizer assembly is provided for organizing and securely holding frequently used cosmetic supplies in a compact space to enhance efficiency. The assembly includes a base tray, an upper tray positionable on the base tray, and a first bowl. The first bowl has a first bowl handle portion. The upper tray has a first upper tray aperture that includes a first upper tray aperture slot portion. Thus, the first upper tray aperture is shaped to receive the first bowl with the first bowl handle portion positioned in the first upper tray aperture slot portion to securely hold the first bowl during use.