Invention Grant
- Patent Title: RNA interference methods using DNA-RNA duplex constructs
- Patent Title (中): 使用DNA-RNA双链体构建的RNA干扰方法
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Application No.: US12911654Application Date: 2010-10-25
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Publication No.: US08372969B2Publication Date: 2013-02-12
- Inventor: Shao-Yao Ying , Shi-Lung Lin
- Applicant: Shao-Yao Ying , Shi-Lung Lin
- Applicant Address: US CA Los Angeles
- Assignee: University of Southern California
- Current Assignee: University of Southern California
- Current Assignee Address: US CA Los Angeles
- Agency: DLA Piper LLP (US)
- Main IPC: C07H21/04
- IPC: C07H21/04

Abstract:
The present invention provides novel compositions and methods for suppressing the function or activity of a targeted gene through a novel intracellular piRNA-mediated RNAi mechanism, using RNA-DNA duplex constructs. The invention further provides novel methods and compositions for generating or producing RNA-DNA duplex agents, whose quantity is high enough to be used for the invention's gene silencing transfection and possibly in therapeutics applications. This improved RNA-polymerase chain reaction (RNA-PCR) method utilizes thermocycling steps of promoter-linked DNA or RNA template synthesis, in vitro transcription and then reverse transcription to bring up the amount of RNA-DNA duplexes up to two thousand folds within one round of the above procedure for using in D-RNAi-directed gene silencing.
Public/Granted literature
- US20110098461A1 Novel RNA Interference Methods Using DNA-RNA Duplex Constructs Public/Granted day:2011-04-28
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