Provided are an imaging optical system in which chromatic aberrations are excellently corrected across the entire screen to realize high optical performance, and an optical apparatus including the imaging optical system. In an imaging optical system of which a back focus is longer than a focal length, a lens unit located in a reduction-conjugate side of an aperture stop includes at least one positive lens. A partial dispersion ratio and an Abbe number of the positive lens are set such that a refractive index, a position, and a power satisfy a suitable relationship.