An anti-rattle track fastener assembly for attaching a fixture to a vehicle that has a track formed therein. The track includes upper walls that form a longitudinally-extending slot. Spaced-apart segments extend from the upper walls into the slot to provide undercut areas in the slot positioned under the segments. Spaced-apart openings are positioned between the segments. The fastener assembly includes a stud that extends from the fixture and is dimensioned to be received within the slot for engaging the spaced-apart segments. The fastener also includes an eccentrically-mounted cam movable between a first position and a second position. When the cam is in the first position, the stud can engage the spaced-apart segments with clearance, and when the cam is in the second position, the stud can tightly engage the spaced-apart segments.