A method of manufacturing a photonic band gap fiber base material includes: a forming step of continuously forming a columnar core glass body 10 and a clad glass body 20 which coats the core glass body to obtain an intermediate base material 110; a hole making step of making holes 30 in the clad glass body 20; an insertion step of inserting in the holes 30 a plurality of bilayer glass rods 40 in which an outer layer 42 which has the same refractive index as the clad glass body coats high refractive index portions 41 having a higher refractive index than a refractive index of the clad glass body 20; and a heating step of heating the intermediate base material 110 and integrating the intermediate base material 110 and the bilayer glass rods 40.