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US08386184B2 Systems and methods for determining an amount of starting reagent using the polymerase chain reaction 有权
使用聚合酶链式反应测定起始试剂的量的系统和方法

Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
Abstract:
Systems and methods for calculating an initial amount of target nucleic acid N0 in a sample are provided. A plurality of fluorescent measurements is received. Each respective fluorescent measurement FSn is taken in a different cycle n in a PCR amplification experiment of the sample. Then, a model for the PCR amplification experiment is computed. For each respective fluorescent measurement, the model comprises a respective equation for Nn, where (i) Nn is the calculated amount of the target nucleic acid in cycle n of the corresponding PCR amplification experiment, and (ii) the equation for Nn is expressed in terms of K and N0, where K is the Michaelis-Menton constant. The model can be refined by adjusting K and N0 until differences between model values Nn and corresponding fluorescent measurements are minimized, thereby calculating the initial amount of a target nucleic acid N0 as the minimized value for N0 for the model.
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