Invention Grant
- Patent Title: Systems and methods for determining an amount of starting reagent using the polymerase chain reaction
- Patent Title (中): 使用聚合酶链式反应测定起始试剂的量的系统和方法
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Application No.: US12196339Application Date: 2008-08-22
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Publication No.: US08386184B2Publication Date: 2013-02-26
- Inventor: Miroslaw Bartkowiak , Richard L. Moore
- Applicant: Miroslaw Bartkowiak , Richard L. Moore
- Applicant Address: US NJ Franklin Lakes
- Assignee: Becton, Dickinson and Company
- Current Assignee: Becton, Dickinson and Company
- Current Assignee Address: US NJ Franklin Lakes
- Agency: Knobbe Martens Olson & Bear LLP
- Main IPC: G06F19/00
- IPC: G06F19/00 ; C12Q1/68 ; C12P19/34

Abstract:
Systems and methods for calculating an initial amount of target nucleic acid N0 in a sample are provided. A plurality of fluorescent measurements is received. Each respective fluorescent measurement FSn is taken in a different cycle n in a PCR amplification experiment of the sample. Then, a model for the PCR amplification experiment is computed. For each respective fluorescent measurement, the model comprises a respective equation for Nn, where (i) Nn is the calculated amount of the target nucleic acid in cycle n of the corresponding PCR amplification experiment, and (ii) the equation for Nn is expressed in terms of K and N0, where K is the Michaelis-Menton constant. The model can be refined by adjusting K and N0 until differences between model values Nn and corresponding fluorescent measurements are minimized, thereby calculating the initial amount of a target nucleic acid N0 as the minimized value for N0 for the model.
Public/Granted literature
- US20090068666A1 Systems and Methods for Determining an Amount of Starting Reagent using the Polymerase Chain Reaction Public/Granted day:2009-03-12
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