Invention Grant
- Patent Title: Methods to fix and detect nucleic acids
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Application No.: US12721550Application Date: 2010-03-10
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Publication No.: US08394588B2Publication Date: 2013-03-12
- Inventor: Thomas Tuschl , John Pena , Cherin Sohn , Sara Hakim , Janos Ludwig , Pavol Cekan
- Applicant: Thomas Tuschl , John Pena , Cherin Sohn , Sara Hakim , Janos Ludwig , Pavol Cekan
- Applicant Address: US NY New York
- Assignee: The Rockefeller University
- Current Assignee: The Rockefeller University
- Current Assignee Address: US NY New York
- Agency: Hoffmann & Baron, LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/48
Abstract:
In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide.
Public/Granted literature
- US20100233706A1 METHODS TO FIX AND DETECT NUCLEIC ACIDS Public/Granted day:2010-09-16
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