The present invention provides a polypeptide as standard for peptide analysis by mass spectrometry comprising at least 16 peptides selected from the group consisting of the peptides of SEQ ID NO: 1 to SEQ ID NO: 22 or variants thereof, together with an artificial protein comprising the polypeptide, a vector comprising a nucleic acid encoding the polypeptide, a kit for proteome analysis, a selection of peptides for calibration an devaluation of mass spectrometers and chromatographs for proteome analysis and uses thereof.