Invention Grant
- Patent Title: Method for the purification of recombinant blood coagulation factor IX enriched in sulfated and/or phosphorylated molecules
- Patent Title (中): 用于纯化富集硫酸化和/或磷酸化分子的重组凝血因子IX的方法
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Application No.: US12037735Application Date: 2008-02-26
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Publication No.: US08399632B2Publication Date: 2013-03-19
- Inventor: Artur Mitterer , Meinhard Hasslacher , Christian Fiedler
- Applicant: Artur Mitterer , Meinhard Hasslacher , Christian Fiedler
- Applicant Address: US IL Deerfield CH Glattpark (Opfikon)
- Assignee: Baxter International Inc.,Baxter Healthcare S.A.
- Current Assignee: Baxter International Inc.,Baxter Healthcare S.A.
- Current Assignee Address: US IL Deerfield CH Glattpark (Opfikon)
- Agency: Morgan, Lewis & Bockius LLP
- Main IPC: C07K1/18
- IPC: C07K1/18 ; C07K14/745
Abstract:
The present invention relates to a method for the purification of rFIX using anion exchange chromatography in the pseudo-affinity mode, wherein said method comprises a wash step with a wash buffer having a salt concentration of more than 200 mM. The purification according to the invention provides a method to enrich rFIX molecules which have been posttranslationally modified by sulfation and/or phosphorylation. The present invention further relates to purified rFIX compositions enriched in monosulfated and/or monophosphorylated rFIX molecules.
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