A method for eliminating chromatic aberration caused by an imaging environment and for testing stability of the imaging environment, and; a chromatic aberration calibration device are disclosed. The method serves to detect errors resulting from environmental factors and human factors and, by using a specific measurement distance and angle, eliminate chromatic aberration caused by such factors. Environmental factors include variations of the image capture device itself; the hue, lightness, and chroma of external light; and the distance and angle between the chromatic aberration calibration device and the image capture device. Human factors include operator-related variations, focusing variations, and hand tremor during measurement. When the same chromatic aberration calibration device and imaging environment are used, the method can show color variations caused by the environmental and human factors in the image capture process and thereby determine whether the imaging environment has the expected stability.