A method and system optimizes the transmission of a downlink reference signal (DLRS) in a wireless communication system that uses orthogonal division multiple access (OFDMA) for the downlink. Each Node-B (base station) is capable of transmitting the DLRS reference symbols in different subframes of the OFDM radio frame and changing both the number and location of the subframes in response to changing network conditions. The network conditions include the number of terminals being served by the Node-B and multiple access interference (MAI) from adjacent Node-Bs.