A method for controlling a multi-port Network Interface Card (NIC) is provided. In a computer using the multi-port NIC with a plurality of NIC ports, a plurality of control options is set into a Basic Input/Output System (BIOS) setup menu, so that a user individually controls the NIC ports. Furthermore, due to the characteristic that after a reference code process in the BIOS restarts a system, the set of hardware becomes effective, an action of controlling the NIC ports is set before a reference code process restarts the system.