A blood transfer assembly comprises of a standard Luer lock style syringe that has a needle and a protective cap in place. The assembly further comprises a blood transfer guide housing that contains a first optional set of barbs that holds the cylindrical end of the syringe. A second set of barbs rigidly hold the needle and prevent the needle being withdrawn from the guide. As the syringe is inserted into the guide the protective cap will pass through the first and second set of barbs until the syringe and the guide come in mechanical contact with each other forming a single assembly.