A computer-implemented system and method is used for facilitating a single instance banking transaction. The method includes the steps of receiving a banking transaction request from a banking account holder at a first banking computer system, the request including at least an account identification and transaction information, generating a single instance banking transaction indicia correlated to the banking transaction request, providing the single instance banking transaction indicia to the banking account holder, receiving the single instance banking transaction indicia at a second banking computer system, and performing the banking transaction request at the second banking computer system.