An apparatus and a method for interference cancellation using a multi-user multiple input multiple output (MU-MIMO) scheme in a single carrier frequency division multiple access (SC-FDMA) system are disclosed. Received data of every terminal are classified into first received data and second received data. The first received data has lower reliability and the second received data has higher reliability than the first received data. The second received data is transmitted to an upper layer. Interference signals are formed with the second received data based on a channel estimation value. The interference signals are removed from the first received data, and the interference signal free first received data is transmitted to the upper layer.