The present invention relates to a temperature controlling apparatus and the temperature controlling method which can arrest foaming and inflation of the air contained in a liquid, and can perform temperature controlling of a liquid of high reliance, and the present invention is constructed to have one or a plurality of sets of nozzles which can suck and discharge a liquid through a tip, and can retain the sucked liquid, a suction and discharge mechanism which can suck and discharge a gas via the each nozzle, a temperature regulator which can maintain one or two or more set predetermined temperatures for a predetermined time regarding one or two or more predetermined temperature controlling regions set in the each nozzle, a movement mechanism which allows relative movement between one or two or more containers which can accommodate a liquid and a nozzle, and a controlling part which instructs the movement mechanism, the suction and discharge mechanism or the temperature regulator to adjust a liquid to be sucked into the nozzle and a liquid amount thereof, a position of the sucked liquid in the nozzle, and temperature controlling of the liquid.