Invention Grant
- Patent Title: Nucleic acid amplification method
- Patent Title (中): 核酸扩增法
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Application No.: US12270706Application Date: 2008-11-13
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Publication No.: US08420323B2Publication Date: 2013-04-16
- Inventor: Hayato Miyoshi , Yoshihide Iwaki , Toshihiro Mori
- Applicant: Hayato Miyoshi , Yoshihide Iwaki , Toshihiro Mori
- Applicant Address: JP Tokyo
- Assignee: FUJIFILM Corporation
- Current Assignee: FUJIFILM Corporation
- Current Assignee Address: JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2007-295337 20071114
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34

Abstract:
An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified using oligonucleotide primers and DNA polymerase. The present invention provides a nucleic acid amplification method which comprises performing incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment, wherein a tag sequence is added at the 5′ end of the first oligonucleotide primer, and the tag sequence is a nucleotide sequence on the template nucleic acid fragment which is present downstream of the sequence which is substantially complementary with the 3′ end region of the first oligonucleotide primer (a region where the first oligonucleotide is annealed to the template nucleic acid).
Public/Granted literature
- US20090162903A1 NUCLEIC ACID AMPLIFICATION METHOD Public/Granted day:2009-06-25
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