Invention Grant
US08426132B2 Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
有权
用标记的探针和3'至5'核酸外切酶活性进行定量扩增
- Patent Title: Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
- Patent Title (中): 用标记的探针和3'至5'核酸外切酶活性进行定量扩增
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Application No.: US12264200Application Date: 2008-11-03
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Publication No.: US08426132B2Publication Date: 2013-04-23
- Inventor: Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
- Applicant: Bin Li , Lei Xi , Yan Wang , Peter B. Vander Horn
- Applicant Address: US CA Hercules
- Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee Address: US CA Hercules
- Agency: Kilpatrick Townsend & Stockton LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
This invention provides methods and kits for performing a quantitative amplification reaction. The method employs a polymerase enzyme and an enzyme having a 3′ to 5′ exonuclease activity that cleaves the 3′ oligonucleotide of the probe.
Public/Granted literature
- US20100159447A1 QUANTITATIVE AMPLIFICATION WITH A LABELED PROBE AND 3' TO 5' EXONUCLEASE ACTIVITY Public/Granted day:2010-06-24
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