A fuel cartridge capable of supplying two fuels to an anode of a fuel cell body without using a pump, a direct methanol fuel cell having the same, and a method of purging a direct methanol fuel cell using the fuel cartridge, fuel cartridge according to one exemplary embodiment comprising a first storage unit having a first port for entrance and exit of a fluid and storing a liquid first fuel; and a second storage unit having a second port for entrance and exit of a fluid and filling a second fuel at a constant pressure, wherein the first fuel is discharged into the first port by the pressure of the second fuel.