Invention Grant
US08436160B2 Isolated DNA fragment of the SPARC human promoter and its use for driving the expression of an heterologous gene in tumor cells
有权
SPARC人启动子的分离DNA片段及其在肿瘤细胞中驱动异源基因表达的用途
- Patent Title: Isolated DNA fragment of the SPARC human promoter and its use for driving the expression of an heterologous gene in tumor cells
- Patent Title (中): SPARC人启动子的分离DNA片段及其在肿瘤细胞中驱动异源基因表达的用途
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Application No.: US12298743Application Date: 2007-04-26
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Publication No.: US08436160B2Publication Date: 2013-05-07
- Inventor: Osvaldo Luis Podhajcer , Eduardo Gustavo Alfredo Cafferata , Maria Veronica Lopez , Diego Luis Viale
- Applicant: Osvaldo Luis Podhajcer , Eduardo Gustavo Alfredo Cafferata , Maria Veronica Lopez , Diego Luis Viale
- Applicant Address: AG Buenos Aires AG Buenos Aires
- Assignee: Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet),Fundacion Instituto Leloir
- Current Assignee: Consejo Nacional de Investigaciones Cientificas y Tecnicas (Conicet),Fundacion Instituto Leloir
- Current Assignee Address: AG Buenos Aires AG Buenos Aires
- Agency: Muncy, Geissler, Olds & Lowe, PLLC
- Priority: ARP060101724 20060428
- International Application: PCT/US2007/010196 WO 20070426
- International Announcement: WO2007/127347 WO 20071108
- Main IPC: C07H21/02
- IPC: C07H21/02 ; C12N15/00 ; A61K48/00
Abstract:
An isolated DNA sequence corresponding to a region of the SPARC gene human promoter from base pair −513 to base pair +35 capable of driving the expression of a heterologous gene of interest, that can be associated to any other promoter sequence, such as radiation responsive sequence, hypoxia responsive sequence and free-radical responsive sequence. The invention also provides constructs and DNA recombinant expression viral vectors, comprising the isolated sequence of the SPARC gene human promoter and at least one heterologous gene operably linked thereto, wherein the promoter sequence drives the expression of the at least one heterologous gene in tumor cells. Pharmaceutical compositions and a method for treating tumors are also provided.
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