A population of seeds wherein substantially all of the seeds in the population have a portion of seed tissue removed by an automated sampling system. Substantially all of the seeds in the population have the presence or absence of at least one transgene in common, and/or substantially all of the seeds in the population have a high recurrent parent allele frequency. The presence or absence of the at least one transgene in the seeds in the population and/or the recurrent parent allele frequency in the seeds in the population is determined by analyzing the portion of seed tissue removed from the seeds by the automated sampling system.