Embodiments of the present invention provide systems and methods for non-invasive, “in-service” AV delay detection and correction. These systems and methods do not modify the audio signal or the video signal, nor do they rely on any metadata to be carried with the audio signal or the video signal via the distribution path. Instead, agents located at various points along the distribution path generate very small signature curves for the audio signal and the video signal and distribute them to a manager via a separate data path other than the distribution path. The manager calculates a measured AV delay caused by the distribution path based on these signature curves, and then optionally corrects the measured AV delay by adjusting an in-line delay in the distribution path.