Invention Grant
- Patent Title: Kits for amplifying and detecting nucleic acid sequences
- Patent Title (中): 用于扩增和检测核酸序列的试剂盒
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Application No.: US11256592Application Date: 2005-10-20
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Publication No.: US08445664B2Publication Date: 2013-05-21
- Inventor: Elazar Rabbani , Jannis Stavrianopoulos , James J. Donegan , Jack Coleman , Marleen Walner
- Applicant: Elazar Rabbani , Jannis Stavrianopoulos , James J. Donegan , Jack Coleman , Marleen Walner
- Applicant Address: US NY Farmingdale
- Assignee: Enzo Diagnostics, Inc.
- Current Assignee: Enzo Diagnostics, Inc.
- Current Assignee Address: US NY Farmingdale
- Agent Anna DiGabriele Petti, Esq.
- Main IPC: C07H21/04
- IPC: C07H21/04

Abstract:
This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention. Post-termination labeling process for nucleic acid sequencing is also disclosed in this invention that is based upon the detection of tagged molecules that are covalently bound to chemically reactive groups provided for chain terminators. A process for producing nucleic acid sequences having decreased thermodynamic stability to complementary sequences is also provided and achieved by this invention. Unique nucleic acid polymers are also disclosed and provided in addition to other novel compositions, kits and the like.
Public/Granted literature
- US20080070241A1 Kits for amplifying and detecting nucleic acid sequences Public/Granted day:2008-03-20
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