Invention Grant
- Patent Title: Method and computer program product for distinguishing and sorting seeds containing a genetic element of interest
- Patent Title (中): 用于区分和分选含有遗传因素的种子的方法和计算机程序产品
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Application No.: US12500847Application Date: 2009-07-10
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Publication No.: US08452445B2Publication Date: 2013-05-28
- Inventor: Steven M. Becker , Steven J. Corak , James L. Hunter , Gary L. Jaehnel , Hugh Lu , James W. Stevenson
- Applicant: Steven M. Becker , Steven J. Corak , Gary L. Jaehnel , Hugh Lu , Catherine Hunter , Catherine Stevenson
- Applicant Address: US IA Johnston
- Assignee: Pioneer Hi-Bred International, Inc.
- Current Assignee: Pioneer Hi-Bred International, Inc.
- Current Assignee Address: US IA Johnston
- Agency: Alston & Bird LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68

Abstract:
A method for distinguishing and sorting seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an photonic emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker. In various other embodiments, the method may comprise associating a red fluorescent protein marker with at least some of the seeds containing a genetic element of interest of the bulk sample, evaluating at least some of the seeds of the bulk sample for the presence of the red fluorescent protein marker using an evaluating device, and sorting the seeds containing a genetic element of interest based on the presence of the red fluorescent protein marker. In some embodiments, the red fluorescent protein marker is discernable when excited by a certain energy and the evaluating step comprises exciting the seeds containing a genetic element of interest with the certain energy and detecting an emission resulting at least in part from the exciting step.
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