A plasma sample collection device includes an elongated blood separation strip having a constant width and a relatively narrower end portion. A support base is located under the plasma separator strip and a cover is positioned superincumbent with respect to the blood separator strip. The cover defines an elongated observation window extending along the length of the plasma separator strip. An oblong collection port is adjacent to but spaced from one end of the observation window. The oblong collection port exposes the separator strip end portion and has a chief axis substantially aligned with the longitudinal axis of the blood separation strip. In one embodiment, the collection port has a teardrop configuration, and in another embodiment it has an obovate-cuneate configuration.