Invention Grant
- Patent Title: Enrichment of a target sequence
- Patent Title (中): 浓缩目标序列
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Application No.: US12671295Application Date: 2008-07-31
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Publication No.: US08455190B2Publication Date: 2013-06-04
- Inventor: Gerassimos Makrigiorgos
- Applicant: Gerassimos Makrigiorgos
- Applicant Address: US MA Boston
- Assignee: Dana-Farber Cancer Institute, Inc.
- Current Assignee: Dana-Farber Cancer Institute, Inc.
- Current Assignee Address: US MA Boston
- Agency: Andrus, Sceales, Starke & Sawall, LLP
- International Application: PCT/US2008/009248 WO 20080731
- International Announcement: WO2009/017784 WO 20090205
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/00 ; C07H21/02 ; C07H21/04

Abstract:
The present invention is directed to methods, compositions, software and devices for enriching low abundance alleles from a sample. The method is based in part on a modified nucleic acid amplification protocol that includes incubating the reaction mixture at a critical denaturing temperature or “Tc”. By employing the present invention the current detection limits of all PCR-based technologies are greatly improved.
Public/Granted literature
- US20100203532A1 Enrichment of a Target Sequence Public/Granted day:2010-08-12
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