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Invention Grant
US08455719B2 Method for increasing seed yield or biomass by expressing RNA binding proteins in transgenic plants 失效
通过在转基因植物中表达RNA结合蛋白来提高种子产量或生物量的方法

Method for increasing seed yield or biomass by expressing RNA binding proteins in transgenic plants
Abstract:
The invention concerns a method for improving growth characteristics of plants by increasing activity in a plant of an RNA-binding protein, which is: (i) a polypeptide having RNA-binding activity and comprising 2 or 3 RNA recognition motifs (RRMs) and a motif having at least 75% identity to motif I: PYEAAVVALPVVVKERLVRILRLGIATRYD (SEQ ID NO: 12) and/or a motif having at least 50% identity to motif II: RFDPFTGEPYKFDP (SEQ ID NO: 13); or (ii) an RBP1 polypeptide having (a) RNA-binding activity; (b) two RRM domains, (c) the following two motifs: (i) KIFVGGL (SEQ ID NO: 41); and (ii) RPRGFGF (SEQ ID NO: 42), allowing for up to three amino acid substitutions and any conservative change in the motifs; and (d) having at least 20% identity to SEQ ID NO: 15. Also provided is transgenic plants introduced with an RNA-binding protein-encoding nucleic acid having improved growth characteristics and constructs useful in the methods.
Abstract(Chinese):

本发明涉及通过增加植物中的RNA结合蛋白的活性来提高植物的生长特性的方法，其是：（i）具有RNA结合活性并包含2或3个RNA识别基序（RRM）的多肽和 基序与基序I具有至少75％的同一性：PYEAAVVALPVVKERLVRILRLGIATRYD（SEQ ID NO：12）和/或与基序II具有至少50％同一性的基序：RFDPFTGEPYKFDP（SEQ ID NO：13）; 或（ii）具有（a）RNA结合活性的RBP1多肽; （b）两个RRM结构域，（c）以下两个基序：（i）KIFVGGL（SEQ ID NO：41）; 和（ii）RPRGFGF（SEQ ID NO：42），允许多达3个氨基酸取代和图案中任何保守的变化; 和（d）与SEQ ID NO：15具有至少20％的同一性。还提供了引入具有改善的生长特性的RNA结合蛋白质编码核酸的转基因植物和在该方法中有用的构建体。

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