An electrowetting display, a pixel array substrate thereof, and an electrowetting display pixel structure thereof are provided. The electrowetting display pixel structure is disposed on a substrate. The electrowetting display pixel structure includes a pixel electrode, an insulating layer and a hydrophobic layer. The pixel electrode is covered with the insulating layer, and the insulating layer is covered with the hydrophobic layer. The hydrophobic layer has at least one flow guiding area, and a flowing path of a fluid medium is determined by a layout of the flow guiding area.