The present invention is to provide an assisted-opening folding knife, which includes a handle having a first positioning groove and a first recess, a blade capable of pivotally springing out of the handle, and a pushing device. A first post on the blade is moved along the first recess while being rotated into the handle, and a second post on the blade is exposed outside the handle while being received in the handle. The pushing device includes a pushing element located in the first positioning groove and a second spring provided between the pushing element and the first positioning groove, so as for the pushing element to move along the groove. Thus, while the blade is rotated into the handle, the first post presses and passes the tip of the pushing element and is then blocked by the pushing element, thereby preventing the blade from springing out of the handle.