The LED lighting bar includes a first conductive wire (10), a second conductive wire (20) parallel to the first conductive wire (10), an insulator (30), at least one LED (40) and a fixing frame (50, 50′). The insulator (30) has an upper layer (31, 31′) and a lower layer (32, 32′), which cover the first conductive wire (10) and the second conductive wire (20), wherein the upper layer (31, 31′) is provided with a penetrating trough (310, 310′) for exposing a first connecting section (11) of the first conductive wire (10) and a second connecting section (21) of the second conductive wire (20). The at least one LED (40) electrically connects on a first electrode (41) of the first connecting section (11) and a second electrode (42) of the second connecting section (21). The fixing frame (50, 50′) is around the at least one LED (40) for fastening.