Invention Grant
US08476038B1 Method for determining capability of a compound for inhibiting a receptor using codon-optimized nucleic acid
有权
用于使用密码子优化的核酸测定化合物抑制受体的能力的方法
- Patent Title: Method for determining capability of a compound for inhibiting a receptor using codon-optimized nucleic acid
- Patent Title (中): 用于使用密码子优化的核酸测定化合物抑制受体的能力的方法
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Application No.: US13761174Application Date: 2013-02-07
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Publication No.: US08476038B1Publication Date: 2013-07-02
- Inventor: Satoshi Inouye , Yuiko Sahara , Junichi Sato
- Applicant: JNC Corporation
- Applicant Address: JP Tokyo
- Assignee: JNC Corporation
- Current Assignee: JNC Corporation
- Current Assignee Address: JP Tokyo
- Agency: Jianq Chyun IP Office
- Priority: JP2009-018339 20090129
- Main IPC: C12Q1/02
- IPC: C12Q1/02 ; C07K14/435 ; C07K21/04 ; C07K14/195 ; C07D241/20
Abstract:
A method for determining a capability of a compound for inhibiting a receptor through a change of intracellular calcium ion when the receptor is activated is provided. Contacting a host cell genetically engineered to express an apo-clytin-II protein generated from a codon-optimized nucleic acid with a test compound, and then determining an amount of light generated. The intracellular calcium ion can be detected much more easily.
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