Invention Grant
US08476081B2 Assay for evaluating affinity and specificity of ligand-albumin binding
有权
用于评估配体 - 白蛋白结合的亲和性和特异性的测定
- Patent Title: Assay for evaluating affinity and specificity of ligand-albumin binding
- Patent Title (中): 用于评估配体 - 白蛋白结合的亲和性和特异性的测定
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Application No.: US12885865Application Date: 2010-09-20
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Publication No.: US08476081B2Publication Date: 2013-07-02
- Inventor: James K. Kranz , Gabrielle C. Todd , Matthew J. Todd
- Applicant: James K. Kranz , Gabrielle C. Todd , Matthew J. Todd
- Applicant Address: BE Beerse
- Assignee: Janssen Pharmaceutica NV
- Current Assignee: Janssen Pharmaceutica NV
- Current Assignee Address: BE Beerse
- Agent Rajiv S. Shah
- Main IPC: G01N33/566
- IPC: G01N33/566
Abstract:
A method for identifying a ligand or compound which binds to albumin comprises the steps of contacting a reaction mixture comprising a site-specific probe and albumin in the presence and the absence of the ligand or compound and measuring either dissociation constant KD, inhibitor concentration IC50 or fluorescence displacement; whereby a change in KD, IC50 and/or fluorescence in the presence of the ligand or compound is indicative of the ligand or compound binds to albumin.
Public/Granted literature
- US20110124120A1 ASSAY FOR EVALUATING AFFINITY AND SPECIFICITY OF LIGAND-ALBUMIN BINDING Public/Granted day:2011-05-26
Information query
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