A tetraploid watermelon inbred 4XASSS4 is disclosed. The invention relates to the seeds and plants of tetraploid watermelon inbred 4XASSS4, the methods of propagating the tetraploid inbred 4XASSS4 through seeds and tissue culture. The invention also relates to methods of producing the triploid seedless watermelon seeds and plants by crossing inbred 4XASSS4 with diploid watermelon inbreds, and to the triploid plants produced therefrom. The invention further relates to the methods of developing new tetraploid lines by using tetraploid inbred 4XASSS4 as breeding material, and to tetraploid watermelon seeds and plants produced therefrom.