The present invention discloses preparation and application of a kind of biodegradable-material-made microsphere vascular embolus that contains liposome-encapsulated cytokines. The present invention is technologically characterized by encapsulation of liposome-wrapped immunocytokines such as IL-2, TNF and interferon by sodium polysaccharide or gelatin, which is extracted from natural alga and is good at biodegradability and biocompatibility. Owing to the encapsulation procedures, cytokine microspheres can take shape measuring 50-75 μm, 75-150 μm, 100-200 μm, 200-300 μm, 200-450 μm, 100-300 μm, 300-500 μm, 500-700 μm, and 700-900 μm. The microsphere preparations are good targeting medication for embolism treatment and immunochemotherapy of animals loaded with human hepatocarcinoma and cervical cancer, as well as human suffering from late-stage or recurrent liver cancer, renal tumors, bladder cancer, colon carcinoma and rectal cancer.