An object of the invention is to provide a multilayer stretched film with barrier property that is free from deterioration of barrier properties attributable to dimensional change caused by moisture absorption, and bending, etc. [Method for Achieving the Object]A multilayer stretched film with barrier property obtained by biaxially stretching a multilayer laminate comprising at least three layers, namely, a polyester-based resin layer (Layer A), a polyamide-based resin layer (Layer B), and a polyester-based resin layer (Layer A), in this order (wherein the two Layers A may be the same or different) to obtain a biaxially stretched multilayer film; and providing a deposition layer (Layer C) on at least one surface of the biaxially stretched multilayer film;both of the Layers A containing a crystalline polyester;Layer B containing 70 to 99 wt % of aliphatic polyamide and 1 to 30 wt % of aromatic polyamide; andLayer C containing an inorganic substance.