Invention Grant
- Patent Title: Methods for ligation independent cloning of DNA
- Patent Title (中): DNA连接独立克隆方法
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Application No.: US11298234Application Date: 2005-12-09
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Publication No.: US08481306B2Publication Date: 2013-07-09
- Inventor: Fenggao Dong , Brian M. Hauge , Christopher Oggero
- Applicant: Fenggao Dong , Brian M. Hauge , Christopher Oggero
- Applicant Address: US MO St. Louis
- Assignee: Monsanto Technology LLC
- Current Assignee: Monsanto Technology LLC
- Current Assignee Address: US MO St. Louis
- Agent James E. Davis; Thomas Kelley
- Main IPC: C12N15/82
- IPC: C12N15/82 ; C12N15/87 ; A01H1/00
Abstract:
The present invention provides methods for assembling DNA molecules in a predetermined order to produce a DNA construct useful in Agrobacterium mediated transformation in plants. The method employs ligation independent cloning of separate DNA elements where one of the DNA elements contains T-DNA borders from Agrobacterium tumefaciens. The invention further provides methods for assembling a construct containing an inverted repeat. Using this approach, DNA constructs are constructed rapidly, efficiently and directionally.
Public/Granted literature
- US20060147961A1 Methods for ligation independent cloning of DNA Public/Granted day:2006-07-06
Information query
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