Invention Grant
- Patent Title: Scaffold polypeptides for heterologous peptide display
- Patent Title (中): 用于异源肽显示的支架多肽
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Application No.: US13243318Application Date: 2011-09-23
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Publication No.: US08481491B2Publication Date: 2013-07-09
- Inventor: Robbie Woodman , Johannes Tsung-Han Yeh , Sophie Laurenson , Paul Ko Ferrigno
- Applicant: Robbie Woodman , Johannes Tsung-Han Yeh , Sophie Laurenson , Paul Ko Ferrigno
- Applicant Address: GB London
- Assignee: Medical Research Council
- Current Assignee: Medical Research Council
- Current Assignee Address: GB London
- Agency: Nixon Peabody LLP
- Agent Mark J. FitzGerald
- Priority: GB0511873.2 20050610; GB0608836.3 20060504
- Main IPC: A61K38/16
- IPC: A61K38/16 ; C07K14/00

Abstract:
The present invention relates to the use of Stefin A as a scaffold protein for the display of inserted peptides, particularly wherein the Stefin A is a human Stefin A. Several mutations are advantageously made in the wild type stefin A sequence to improve it as a scaffold; preferably the Stefin A comprises a heterologous peptide insertion at the Leu 73 site. Furthermore, preferably the scaffold protein comprises a V48D mutation; preferably the scaffold protein comprises a G4W mutation. Preferably the scaffold comprises Leu73, V48D and G4W mutations. The invention also relates to the scaffold proteins themselves, in particular a stefin A polypeptide having the Leu73, V48D and G4W mutations, such as shown as SEQ ID NO: 1. The invention also relates to a method for identifying binding proteins and to peptide A (RLNKPLPSLPV, SEQ ID NO: 30) and its use in treating yeast infections.
Public/Granted literature
- US20120190819A1 SCAFFOLD POLYPEPTIDES FOR HETEROLOGOUS PEPTIDE DISPLAY Public/Granted day:2012-07-26
Information query
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