The disclosure is a device and a method for receiver-equalizer calibration, in which the device includes an adaptive filter, a Clock Data Recovery (CDR) unit, an adaptive control unit and a run length encoding unit. The adaptive filter receives a channel signal, calibrates the channel signal according to a filter control signal and compensates the channel signal to obtain a compensative signal. The CDR unit receives the compensative signal to generate a sampling clock signal, a data signal and a transition sampling signal. The run length encoding unit receives the data signal and run-length encodes the data signal to generate first code data and second code data. The adaptive control unit receives the first code data, the second code data, the data signal and the transition sampling signal, and performs weight calculation to adjust the filter control signal.