Invention Grant
US08486650B2 Method of detecting serum gamma-glutamyl transferase isoforms in a biological fluid
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检测生物体液中血清γ-谷氨酰转移酶同种型的方法
- Patent Title: Method of detecting serum gamma-glutamyl transferase isoforms in a biological fluid
- Patent Title (中): 检测生物体液中血清γ-谷氨酰转移酶同种型的方法
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Application No.: US12665952Application Date: 2008-06-24
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Publication No.: US08486650B2Publication Date: 2013-07-16
- Inventor: Aldo Paolicchi , Alfonso Pompella , Maria Franzini , Renata Barsacchi , Michele Emdin , Emilia Bramanti
- Applicant: Aldo Paolicchi , Alfonso Pompella , Maria Franzini , Renata Barsacchi , Michele Emdin , Emilia Bramanti
- Applicant Address: IT Pisa
- Assignee: Universita' di Pisa
- Current Assignee: Universita' di Pisa
- Current Assignee Address: IT Pisa
- Agency: Ladas & Parry, LLP
- Priority: ITTO2007A0455 20070625
- International Application: PCT/IB2008/052499 WO 20080624
- International Announcement: WO2009/001290 WO 20081231
- Main IPC: C12Q1/48
- IPC: C12Q1/48
Abstract:
A method is described for detecting the gamma-glutamyl transferase enzyme isoforms (GGT, EC 2.3.2.2) in a sample of biological fluid, such as for example plasma or serum. The method comprises an HPLC separation step of the sample proteins based on the molecular size and a second step for detecting the GGT isoforms by post-column reaction with a GGT enzyme substrate capable of generating a detectable final product, preferably by spectrophotometric or fluorimetric means. The GGT isoforms can be separated by ultra-centrifugation, thereby obtaining three enzymatic isoforms characterized by molecular weights of approximately 2000, 940, and 140 KDa, respectively.
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